Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCK1 (Ki = 220 nM) and ROCK2 (Ki = 300 nM) by competing with ATP for binding to the catalytic site (Davies et al.; Ishizaki et al.). MAINTENANCE AND SELF-RENEWAL · Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency (Watanabe et al.). · Improves embryoid body formation using forced-aggregation protocols (Ungrin et al.). · Increases the survival of cryopreserved single human ES cells after thawing (Li et al.). · Blocks apoptosis of mouse ES-derived neural precursors after dissociation and transplantation (Koyanagi et al.). REPROGRAMMING · Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, RepSox, Forskolin, SP600125, Gö6983 and Valproic Acid (Hu et al.). DIFFERENTIATION · Improves survival of human ES cell monolayers at the initiation of differentiation protocols (Rezania et al.) Alternative Names Not applicable Cell Type 多能干细胞, 神经元 Species 人, 小鼠, 大鼠, 非人灵长类动物, 其他 Application 分化, 扩增, 细胞球培养, 维持培养, 重编程 Area of Interest 干细胞生物学 CAS Number 129830-38-2 Chemical Formula C₁₄H₂₁N₃O · 2HCl Molecular Weight 320.3 g/mol Purity ≥ 98% Pathway RHO/ROCK Target ROCK1, ROCK2